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Title: The effects of genetic manipulation of putrescine biosynthesis on transcription and activities of the other polyamine biosynthetic enzymes
Author: Page, Andrew F.; Mohapatra, Sridev; Minocha, Rakesh; Minocha, Subhash C.
Source: Physiologia Plantarum. 129: 707-724.
Description: We have studied the effects of overproduction of putrescine (Put) via transgenic expression of a mouse ornithine decarboxylase (ODC) gene on the expression of native genes for four enzymes involved in polyamine biosynthesis in hybrid poplar (Populus nigra x maximowiczii) cells. An examination of the transcript levels of arginine decarboxylase (ADC), ODC, S-adenosylmethionine decarboxylase (SAMDC) and spermidine synthase (SPDS), as well as their enzyme activities (except SPDS), showed that the expression of different members of the SAMDC and SPDS gene families was affected differently in response to alteration of the cellular Put content. It was further observed that there was a strong correlation between transcript levels and the activity of the respective enzyme in the cells. Moreover, there was no feedback inhibition of the expression of the native ODC or the ADC genes or their enzyme activities by increased Put in the cells.
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Page, Andrew F.; Mohapatra, Sridev; Minocha, Rakesh; Minocha, Subhash C. 2007. The effects of genetic manipulation of putrescine biosynthesis on transcription and activities of the other polyamine biosynthetic enzymes. Physiologia Plantarum. 129: 707-724.
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