You are here: Home
/ Publication Information
Title: Identification of a small heat-shock protein associated with a ras-mediated signaling pathway in ectomycorrhizal symbiosis
Author: Hiremath, Shiv; Lehtoma, Kirsten; Podila, Gopi K.
Source: Res. Pap. NRS-7. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station. 8 p.
Description: Initiation, development, and establishment of a functional ectomycorrhiza involve a series of biochemical events mediated by a number of genes from the fungus as well as the host plant. We have identified a heat shock protein gene from Laccaria bicolor (Lbhsp) that appears to play a role in these events. The size and characteristics of Lbhsp suggest that it belongs to the family of small heat-shock proteins described in the literature. Nucleotide sequencing of an almost full length cDNA indicated that the Lbhsp mRNA is about 611 nucleotides long and codes for a single protein of ~ 17 kDa. Isolation and characterization of the Lbhsp gene showed that it was made up of three exons separated by two small introns. Southern analysis suggested that the L. bicolor genome contains at least two copies of the Lbhsp gene. Temporal expression analyses revealed that the gene is expressed within 4 to 12 hours after interaction with red pine roots. The yeast two-hybrid studies showed that the Lbhsp was closely associated with the ras gene (Lbras) described earlier. The data suggest that Lbhsp may play a supporting role in ras-mediated mycorrhizal signaling pathways during various stages of ectomycorrhizal development.
Keywords: mycorrhizal fungi, heat-shock protein, symbiosis, ras-mediated
View or Print this Publication (276 KB)
- We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
- This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
- This publication may be available in hard copy. Check the Northern Research Station web site to request a printed copy of this publication.
- Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact Sharon Hobrla, email@example.com if you notice any errors which make this publication unusable.
Hiremath, Shiv; Lehtoma, Kirsten; Podila, Gopi K. 2009. Identification of a small heat-shock protein associated with a ras-mediated signaling pathway in ectomycorrhizal symbiosis. Res. Pap. NRS-7. Newtown Square, PA: U.S. Department of Agriculture, Forest Service, Northern Research Station. 8 p.
Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility